›› 2012, Vol. 24 ›› Issue (1): 68-70.doi: 10.3969/j.issn.1004-616x.2012.01.016

• 检测研究 • Previous Articles     Next Articles

Evaluation of mutagenicity and anti-mutagenicity of aloe-emodin and aloe extract

YUAN Ya，CHEN Wei，ZHANG Li-shi   

  1. Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China
  • Received:2011-07-29 Revised:2011-09-14 Online:2012-01-30 Published:2012-01-30
  • Contact: ZHANG Li-shi

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Abstract: OBJECTIVE: To evaluate the mutagenicity and anti-mutagenicity of aloe-emodin and aloe extract by in vitro micronucleus test in L5178Y cells. METHODS：For 4 concentration of both aloe-emodin and aloe extract，mutagenicity and anti-mutagenicity groups were set up，as well as solvent control，positive control and anti-mutagenicity control. The L5178Y cells were treated respectively for 12 h，and in vitro micronucleus test was analyzed by routine method. RERULTS：Aloe-emodin showed mutagenic effect at 6.67 μg/ml. Micronucleus rate at this dose showed a significant difference compared with the control group (P＜0.05)，but aloe extract showed non-mutagenic effect in this study. Within a certain dose range，both aloe-emodin(0.22 μg/ml) and aloe extract(20 μg/ml) could exert antagonistic effect on the increase of micronucleus rate induced by MMS，showing a significant difference compared with the control group (P＜0.01). CONCLUSION：Under our experimental conditions，aloe-emodin showed weak mutagenic effect， while aloe extract was not found to have mutagenic effect. Both of them displayed anti-mutagenic activity.

Key words: L5178Y, in vitro micronucleus test, aloe, mutagenicity, anti-mutagenicity